Achour A, Picard O, Mbika J P, Willer A, Snart R, Bizzini B, Carelli C, Burny A, Zagury D
Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, Paris, France.
Vaccine. 1993;11(7):609-701. doi: 10.1016/0264-410x(93)90251-r.
Cytotoxic T cells are the main antigen-specific effector cells of the cellular immune system and MHC class I restricted cytotoxic T-lymphocyte (CTL) responses in mice, acting against the HIV-1 envelope protein, are known to be predominantly directed against an amino acid sequence in the third hypervariable domain. We have investigated the epitope specificity of anti-HIV-1 CTL in healthy human volunteers inoculated with a recombinant vaccinia expressing the HIV-1 gp160 envelope gene. Their isolated lymphocytes were stimulated in vitro with autologous HIV-1 infected cells. Our results show that immunization with recombinant virus is able to generate virus-specific CTLs to the HIV-1 gp160 envelope protein and to a 15-residue synthetic peptide corresponding to a highly variable region of the envelope p18(IIIB). The CTL response was restricted by class I MHC molecules HLA-A2 and A3 that commonly occur in the human population.
细胞毒性T细胞是细胞免疫系统主要的抗原特异性效应细胞，在小鼠中，MHC I类限制性细胞毒性T淋巴细胞（CTL）应答针对HIV-1包膜蛋白，已知主要针对第三个高变区的氨基酸序列。我们研究了接种表达HIV-1 gp160包膜基因的重组痘苗病毒的健康人类志愿者体内抗HIV-1 CTL的表位特异性。用自体HIV-1感染细胞在体外刺激他们分离出的淋巴细胞。我们的结果表明，用重组病毒免疫能够产生针对HIV-1 gp160包膜蛋白以及对应包膜p18（IIIB）高变区的15个氨基酸残基合成肽的病毒特异性CTL。CTL应答受人群中常见的I类MHC分子HLA-A2和A3的限制。
