Achour A, Lemhammedi S, Picard O, M'Bika J P, Zagury J F, Moukrim Z, Willer A, Beix F, Burny A, Zagury D
Université Pierre et Marie Curie, Paris, France.
AIDS Res Hum Retroviruses. 1994 Jan;10(1):19-25. doi: 10.1089/aid.1994.10.19.
Cytotoxic T cell determinants should be an important component of an anti-human immunodeficiency virus (HIV) vaccine. The epitopes of proteins can be defined with short synthetic peptides for class I-restricted CTLs. An immunodominant CTL epitope from the HIV-1 IIIB envelope protein gp160 comprising 15 amino acids (residues 315-329: RIQRGPGRAFVTIGK) (P18IIIB) has been identified that is recognized by class I MHC molecule H-2d-restricted murine CD8+ CTLs. We have investigated the epitope specificity of anti-HIV-1 CTLs in immunized individuals and we found that the CTL response was restricted by more than one class I MHC molecule, including HLA-A2 and HLA-A3. In the present work, we also show that the response against P18IIIB peptide is restricted by the HLA-A11 molecule in an individual immunized by vaccinia virus expressing gp160 protein. This peptide could thus be recognized in association with different HLA class I allotypes. This work has implications for vaccine strategies, using the P18 peptide.
细胞毒性T细胞决定簇应是抗人类免疫缺陷病毒（HIV）疫苗的重要组成部分。蛋白质的表位可用针对I类限制性CTL的短合成肽来确定。已鉴定出一种来自HIV-1 IIIB包膜蛋白gp160的免疫显性CTL表位，其由15个氨基酸组成（第315 - 329位残基：RIQRGPGRAFVTIGK）（P18IIIB），可被I类MHC分子H-2d限制性小鼠CD8 + CTL识别。我们研究了免疫个体中抗HIV-1 CTL的表位特异性，发现CTL反应受不止一种I类MHC分子限制，包括HLA-A2和HLA-A3。在本研究中，我们还表明，在接种表达gp160蛋白的痘苗病毒的个体中，针对P18IIIB肽的反应受HLA-A11分子限制。因此，该肽可与不同的HLA I类同种异型结合被识别。这项工作对使用P18肽的疫苗策略具有启示意义。
