Salford L G, Persson B R, Brun A, Ceberg C P, Kongstad P C, Mir L M
Department of Neurosurgery, Lund University Hospital, Sweden.
Biochem Biophys Res Commun. 1993 Jul 30;194(2):938-43. doi: 10.1006/bbrc.1993.1911.
The potentials of in vivo electropermeabilization in combination with bleomycin in brain tumor treatment have been explored. In the brain of normal Fischer 344 rats, 2 electrodes were placed 5 mm apart. Electropermeabilization was performed with 8 to 12 exponential 400 V pulses with a time constant of 325 microseconds. Some animals were given bleomycin i.v., 1mg/kg b.w., 4 minutes before electric pulses delivery. No adverse effects were recorded during the observation of the animals during the following month. The effect of bleomycin and electropermeabilization upon tumour growth was studied in rats with glioma cells (RG2) implanted in the head of the right caudate nucleus. Treatment was given at different time intervals after the implantation of tumor cells and the effect upon survival was studied. Bleomycin alone did not prolong the survival of the animals. On the contrary, bleomycin plus electropermeabilization on the 10th, 11th or 12th day after inoculation increased the survival time to almost double that of untreated animals. We conclude that this treatment may be of value in brain tumour therapy.
已对体内电穿孔与博来霉素联合用于脑肿瘤治疗的潜力进行了探索。在正常的Fischer 344大鼠脑中，将两个电极相距5毫米放置。用8至12个指数为400 V的脉冲进行电穿孔，时间常数为325微秒。在电脉冲施加前4分钟，给一些动物静脉注射1mg/kg体重的博来霉素。在接下来的一个月观察动物期间未记录到不良反应。在右尾状核头部植入胶质瘤细胞（RG2）的大鼠中研究了博来霉素和电穿孔对肿瘤生长的影响。在肿瘤细胞植入后的不同时间间隔进行治疗，并研究对生存的影响。单独使用博来霉素并不能延长动物的生存期。相反，在接种后第10、11或12天进行博来霉素加电穿孔治疗可使生存时间增加至几乎是未治疗动物的两倍。我们得出结论，这种治疗方法可能在脑肿瘤治疗中具有价值。
