Knapp L, Lawton A, Oakley B, Wong L, Zhang C
Department of Biology, University of Michigan, Ann Arbor 48109, USA.
Differentiation. 1995 Jun;58(5):341-9. doi: 10.1046/j.1432-0436.1995.5850341.x.
Cytokeratins in taste buds were immunocytochemically evaluated with monoclonal antibodies. In each of six different epithelial sites in the rat oral cavity, intragemmal cells of taste buds were immunoreactive for keratin polypeptides 8, 18, and 19, as well as for keratin 7, which has not been previously reported in taste buds. Keratin-18-like immunoreactivity was present in fewer than half of the intragemmal cells, whereas all intragemmal cells were immunopositive for keratins 7, 8, and 19. Apart from some salivary duct cells, no other cells in the tongue were immunoreactive for any of these four keratins. Morphological and immunocytochemical profiles indicate that taste buds are islets of simple epithelium embedded in an expanse of stratified squamous epithelium. These simple epithelial cells and their keratins are nerve-dependent, since denervation eliminated all four keratins and replaced elongated taste cells of the vallate papilla with stratified squamous epithelium. We conclude that antibodies against keratins 7, 8, or 19 are useful markers for intragemmal cells in studies of taste bud development, degeneration, regeneration, turnover and tissue culture.
运用单克隆抗体对味蕾中的细胞角蛋白进行免疫细胞化学评估。在大鼠口腔六个不同的上皮部位，味蕾内的细胞对角蛋白多肽8、18和19呈现免疫反应性，同时也对细胞角蛋白7呈现免疫反应性，而细胞角蛋白7在味蕾中此前尚未见报道。细胞角蛋白18样免疫反应性存在于不到一半的味蕾内细胞中，而所有味蕾内细胞对角蛋白7、8和19均呈免疫阳性。除了一些涎腺导管细胞外，舌内没有其他细胞对这四种角蛋白中的任何一种呈现免疫反应性。形态学和免疫细胞化学特征表明，味蕾是嵌入复层鳞状上皮中的单层上皮小岛。这些单层上皮细胞及其角蛋白依赖神经，因为去神经支配消除了所有这四种角蛋白，并使轮廓乳头的细长味细胞被复层鳞状上皮所取代。我们得出结论，针对细胞角蛋白7、8或19的抗体在味蕾发育、退化、再生、更新及组织培养研究中是味蕾内细胞的有用标记物。
