双链PCR产物无需中间片段纯化的直接测序；用绿豆核酸酶消化。 - Suppr

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超能文献

Direct sequencing of double-stranded PCR products without intermediate fragment purification; digestion with mung bean nuclease.

作者信息

Dowton M, Austin A D

机构信息

Department of Crop Protection, University of Adelaide, Australia.

出版信息

Nucleic Acids Res. 1993 Jul 25;21(15):3599-600. doi: 10.1093/nar/21.15.3599.

DOI:10.1093/nar/21.15.3599

PMID:7688458

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331479/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20d9/331479/de611ca071a3/nar00064-0256-a.jpg

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引用本文的文献

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本文引用的文献

Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.动物线粒体DNA进化的动力学：用保守引物进行扩增和测序

Proc Natl Acad Sci U S A. 1989 Aug;86(16):6196-200. doi: 10.1073/pnas.86.16.6196.

Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method.哺乳动物基因中的点突变分析：总RNA的快速制备、cDNA的PCR扩增以及一种新方法的Taq测序

Biotechniques. 1989 May;7(5):494-6, 498-9.

Activation of S1 nuclease at neutral pH.在中性pH条件下S1核酸酶的激活

Nucleic Acids Res. 1992 Sep 25;20(18):4932. doi: 10.1093/nar/20.18.4932.

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