Raghunath P N, Sidhu G S, Coon H C, Liu K, Srikantan V, Maheshwari R K
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
J Biol Regul Homeost Agents. 1993 Jan-Mar;7(1):22-30.
We have studied the effects of interferon (IFNs) alpha and gamma on the regulation and expression of laminin (LMN) and a 32 kD laminin binding protein (LPB-32) in cultured human umbilical vein endothelial cells (HUVEC) and human foreskin fibroblast (FS-4) cells. We show that IFNs increased immunofluorescent staining for LMN and LPB-32. In HUVEC, B1 and B2 chain immunoprecipitated proteins were enhanced in the extracellular (released) fraction by IFN-alpha, but were decreased by IFN-gamma. In intracellular (cell-associated) fractions, both B chains were increased, especially by IFN-gamma. In situ hybridization of FS-4 cells demonstrated increased B2 chain mRNA in the presence of IFNs. Reverse transcription-polymerase chain reaction amplification (RT-PCR) indicated that B1 chain mRNA was increased by both IFNs in HUVEC, and by IFN-gamma in FS-4. The increased synthesis of LMN and LBP-32 may be important in promoting wound healing and angiogenesis.
我们研究了α干扰素（IFN）和γ干扰素对培养的人脐静脉内皮细胞（HUVEC）和人包皮成纤维细胞（FS-4）中层粘连蛋白（LMN）和一种32kD层粘连蛋白结合蛋白（LPB-32）的调节和表达的影响。我们发现，干扰素增加了LMN和LPB-32的免疫荧光染色。在HUVEC中，α干扰素使细胞外（释放）部分中B1和B2链免疫沉淀蛋白增加，而γ干扰素则使其减少。在细胞内（细胞相关）部分，两条B链均增加，尤其是γ干扰素作用下。FS-4细胞的原位杂交显示，在干扰素存在的情况下B2链mRNA增加。逆转录-聚合酶链反应扩增（RT-PCR）表明，α干扰素和γ干扰素均使HUVEC中的B1链mRNA增加，γ干扰素使FS-4中的B1链mRNA增加。LMN和LBP-32合成的增加可能在促进伤口愈合和血管生成中起重要作用。
