Calzà L, Giardino L, Ceccatelli S
Institute of Human Physiology, University of Cagliari, Italy.
Neuroreport. 1993 Jun;4(6):627-30. doi: 10.1097/00001756-199306000-00006.
Nitric oxide synthesizing (NOS) enzyme has been localized in distinct neural populations, including the hypothalamic paraventricular nucleus (PVN). Using in situ hybridization, we have investigated possible changes in NOS mRNA in the PVN of young and old rats after 2 h of immobilization stress. In old rats a 3-fold increase in NOS mRNA was observed in the PVN immediately after the stressor, whereas in young rats the increase reached significance when animals were killed 3 h after the end of the stress procedure. The present results suggest that nitric oxide in the PVN may be involved in the stress response. The differences observed in NOS mRNA between young and old rats could be related to an age-dependent different responsiveness to stressful stimuli.
一氧化氮合成（NOS）酶已定位在不同的神经群体中，包括下丘脑室旁核（PVN）。我们采用原位杂交技术，研究了固定应激2小时后年轻和老年大鼠PVN中NOS mRNA的可能变化。在老年大鼠中，应激源作用后立即观察到PVN中NOS mRNA增加了3倍，而在年轻大鼠中，应激程序结束3小时后处死动物时，这种增加才具有统计学意义。目前的结果表明，PVN中的一氧化氮可能参与应激反应。年轻和老年大鼠之间观察到的NOS mRNA差异可能与年龄依赖性的对应激刺激的不同反应性有关。
