Tatsumi E, Yoneda N, Kawano S, Yamaguchi N
Department of Laboratory Medicine, Kobe University School of Medicine.
Rinsho Byori. 1993 Apr;41(4):377-83.
One of 8 to 12 pre-B ALL cells co-express CD13 and CD33 antigens, but such blasts do not express myeloperoxidase (MPO) even on electronmicroscopy or mRNA. MPO+ pre-B ALL is extremely rare (1/50-1/100), however a cell-line (Tahr87) was established in culture. In contrast, T-lineage blasts express CD13/33 antigens regularly in the pro-thymic stage (CD7+ 5+ 2+ 3- 4- 8- or more immature), and a limited expression of MPO is rather commonly detected particularly in recurrences. The co-expression of CD3 epsilon/MPO or CD3 epsilon/delta/MPO mRNA has been demonstrated. Thus, the regulation of MPO expression is of utmost importance in interpreting the phenotypes of leukemia/lymphoma. While testing the effects of several cytokines on MPO expression, IFN-gamma was found to suppress the gene expression of MPO in HL60 cells. This suppression was not accompanied by differentiation, termination of proliferation or reduction of cytochemical MPO+ cells, and was reversible. Among 22 cases of M1 AML blasts, 8 cases were HLA-DR(-). DR antigen was induced by the presence of a mixture of IFN-gamma, TNF-alpha and TPA in 4 cases, but not in the other 4 cases. The blasts of the latter 4 cases were always CD34(-), CD7(-) and CD45RA-/RO+, and constituted a distinct M1 subset which has not previously been reported.
8至12个前B细胞急性淋巴细胞白血病细胞中有1个会共同表达CD13和CD33抗原，但即使在电子显微镜下或mRNA水平，此类原始细胞也不表达髓过氧化物酶（MPO）。MPO阳性的前B细胞急性淋巴细胞白血病极为罕见（1/50 - 1/100），不过已在培养中建立了一个细胞系（Tahr87）。相比之下，T系原始细胞在胸腺前阶段（CD7 + 5 + 2 + 3 - 4 - 8 - 或更不成熟）会规律性地表达CD13/33抗原，并且相当常见地检测到MPO有有限表达，尤其是在复发时。已证实存在CD3ε/MPO或CD3ε/δ/MPO mRNA的共同表达。因此，MPO表达的调控对于解释白血病/淋巴瘤的表型至关重要。在测试几种细胞因子对MPO表达的影响时，发现干扰素-γ可抑制HL60细胞中MPO的基因表达。这种抑制不伴有分化、增殖终止或细胞化学MPO阳性细胞减少，并且是可逆的。在22例M1急性髓系白血病原始细胞中，8例HLA-DR（-）。4例中干扰素-γ、肿瘤坏死因子-α和佛波酯混合物的存在诱导了DR抗原表达，但另外4例未诱导。后4例的原始细胞始终为CD34（-）、CD7（-）和CD45RA - /RO +，构成了一个此前未报道过的独特M1亚群。
