在一例表达CD7的t(8;21)阳性急性髓系白血病中髓系抗原和髓过氧化物酶的不一致表达

Discordant expression of myeloid antigens and myeloperoxidase in a case of t(8;21) positive AML expressing CD7.

作者信息

Hirai K, Torimoto Y, Moriichi K, Sato K, Ohnishi K, Taya N, Kohgo Y

机构信息

Third Department of Internal Medicine, Asahikawa Medical College, Japan.

出版信息

Int J Hematol. 1999 Jul;70(1):30-5.

PMID:10446492

Abstract

We describe a case of acute myeloid leukemia (AML) showing myeloperoxidase (MPO)-positive and myeloid antigens negative. Although the leukemic cells showed few granules in May-Grünwald Giemsa staining, cytochemical MPO staining revealed that most of the blast cells strongly reacted with MPO. The leukemic cells did not express myeloid antigens (CD13, CD33), nor B-lymphoid or T-lymphoid antigens on the cell surface using flow cytometry, however. The cells did express CD34 and CD7. Discordant expression of MPO and myeloid antigens was also confirmed by electron microscopic MPO staining and by immunocytochemistry using a streptoavidin-biotin alkaline phosphatase labeling technique. Cytogenetic studies showed 46, XX, t(8;21) (q22;q22), del (9) (q22) in the bone marrow cells. In addition, AML1/ETO chimeric mRNA was detected from these cells. We summarize eight reported cases of MPO positive and myeloid antigens negative AML. Five of nine cases including our case had the same chromosomal abnormality of t(8;21) (q22;q22) and showed better prognosis than the other cases.

摘要

我们描述了一例急性髓系白血病（AML），其髓过氧化物酶（MPO）呈阳性而髓系抗原呈阴性。尽管白血病细胞在May-Grünwald Giemsa染色中显示颗粒较少，但细胞化学MPO染色显示大多数原始细胞与MPO强烈反应。然而，使用流式细胞术检测发现白血病细胞在细胞表面不表达髓系抗原（CD13、CD33），也不表达B淋巴细胞或T淋巴细胞抗原。这些细胞确实表达CD34和CD7。通过电子显微镜MPO染色以及使用链霉抗生物素蛋白-生物素碱性磷酸酶标记技术的免疫细胞化学也证实了MPO和髓系抗原的不一致表达。细胞遗传学研究显示骨髓细胞中存在46, XX, t(8;21) (q22;q22), del (9) (q22)。此外，从这些细胞中检测到了AML1/ETO嵌合mRNA。我们总结了8例已报道的MPO阳性而髓系抗原阴性的AML病例。包括我们的病例在内，9例中有5例具有相同的t(8;21) (q22;q22)染色体异常，且预后比其他病例更好。