Linacero R, Vázquez A M
Departamento de Génetica, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Spain.
Mutat Res. 1993 Aug;302(4):201-5. doi: 10.1016/0165-7992(93)90105-5.
We studied the genetic variation generated during in vitro culture of rye Secale cereale L. We analyzed the progenies of four generations of the plants regenerated from immature embryo cultures. A high frequency of mutant plants was observed, 50.75%, this frequency was genotype dependent. Other characteristics typical of somaclonal variation were also observed: the obtaining of dominant mutations, the presence of more than one mutation per plant, the obtaining of homozygous mutants and a high rate of mutation of particular loci. In some cases transposable elements could be implicated. We postulate that tissue culture could induce mutations as well as select particular cell types and so increase the appearance of special mutants.
我们研究了黑麦（Secale cereale L.）体外培养过程中产生的遗传变异。我们分析了从未成熟胚培养再生的四代植株的后代。观察到突变植株的频率很高，为50.75%，该频率取决于基因型。还观察到体细胞克隆变异的其他典型特征：获得显性突变、每株植物存在多个突变、获得纯合突变体以及特定基因座的高突变率。在某些情况下，可能涉及转座因子。我们推测组织培养可以诱导突变以及选择特定的细胞类型，从而增加特殊突变体的出现。
