Hanski C, Drechsler K, Hanisch F G, Sheehan J, Manske M, Ogorek D, Klussmann E, Hanski M L, Blank M, Xing P X
Klinikum Steglitz, Freien Universität Berlin, Germany.
Cancer Res. 1993 Sep 1;53(17):4082-8.
The mucin carbohydrate epitope sialyl-Le(x), detected with the monoclonal antibody AM-3, is strongly overexpressed in > 90% of human colon carcinomas. We show here that in colon carcinoma one of the mucin cores bearing the sialyl-Le(x) group is MUC-1, whereas sialyl-Le(x) present in normal colon is not detectable on MUC-1. The amounts of MUC-1 core detectable with the monoclonal antibody BC3 in extracts of tumor tissue are 60-180% of those in normal tissue. Two other carbohydrate epitopes located on MUC-1 in mucins from normal and tumor tissue have also been characterized. In contrast to sialyl-Le(x), their expression on MUC-1 is variable and does not correlate with the malignant transformation of colonic mucosa. The transfer of the sialyl-Le(x) group onto the MUC-1 core contributes to the colon carcinoma-associated overexpression of the sialyl-Le(x) epitope.
用单克隆抗体AM-3检测到的黏蛋白碳水化合物表位唾液酸化路易斯寡糖（sialyl-Le(x)），在90%以上的人类结肠癌中强烈过表达。我们在此表明，在结肠癌中，带有唾液酸化路易斯寡糖基团的一种黏蛋白核心是MUC-1，而正常结肠中存在的唾液酸化路易斯寡糖在MUC-1上无法检测到。用单克隆抗体BC3在肿瘤组织提取物中检测到的MUC-1核心量是正常组织中的60%-180%。正常组织和肿瘤组织黏蛋白中位于MUC-1上的另外两个碳水化合物表位也已得到表征。与唾液酸化路易斯寡糖不同，它们在MUC-1上的表达是可变的，且与结肠黏膜的恶性转化无关。唾液酸化路易斯寡糖基团转移到MUC-1核心上导致了与结肠癌相关的唾液酸化路易斯寡糖表位的过表达。
