Iida S, Tsuiji H, Nemoto Y, Sano Y, Reddish M A, Irimura T
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
Oncol Res. 1998;10(8):407-14.
The levels of mRNA corresponding to the MUC1, MUC2, MUC5AC, MUC5B, and MUC6 genes were determined in 19 human colon adenocarcinoma cell lines by the reverse transcriptase-polymerase chain reaction method using specific primers in an attempt to correlate to the levels of cell surface carbohydrate epitopes. All 19 cell lines expressed MUC1 and MUC5B mRNA, whereas MUC2, MUC5AC, or MUC6 mRNA were only detected in 8, 3, or 2 of 19 cell lines, respectively. Sialyl Lewis a carbohydrates, identified by the monoclonal antibody (mAb) CA19-9, and sialyl Lewis X carbohydrates. identified by mAb KM93, were observed, with most of the cell lines expressing multiple mucin core polypeptide genes but with few cell lines expressing only MUC1 and MUC5B. Sialyl Tn epitopes identified by mAb B195.3R11 and by mAb TKH-2 were strongly expressed on both of two MUC6-positive cells, whereas only a small portion of MUC6-negative cells expressed these epitopes. Strict correlation between mucin gene expression and any carbohydrate epitopes examined was not observed.
采用逆转录-聚合酶链反应方法，使用特异性引物，在19种人结肠腺癌细胞系中测定了与MUC1、MUC2、MUC5AC、MUC5B和MUC6基因相对应的mRNA水平，试图将其与细胞表面碳水化合物表位水平相关联。所有19种细胞系均表达MUC1和MUC5B mRNA，而MUC2、MUC5AC或MUC6 mRNA分别仅在19种细胞系中的8种、3种或2种中检测到。观察到由单克隆抗体（mAb）CA19-9识别的唾液酸化路易斯a碳水化合物和由mAb KM93识别的唾液酸化路易斯X碳水化合物，大多数细胞系表达多种粘蛋白核心多肽基因，但只有少数细胞系仅表达MUC1和MUC5B。由mAb B195.3R11和mAb TKH-2识别的唾液酸化Tn表位在两个MUC6阳性细胞上均强烈表达，而只有一小部分MUC6阴性细胞表达这些表位。未观察到粘蛋白基因表达与所检测的任何碳水化合物表位之间存在严格的相关性。
