Chen P J, Hwang L H, Chang L Y, Kao J H, Chen D S
Graduate Institute of Clinical Medicine, National Taiwan University Hospital, Taipei.
Gastroenterol Jpn. 1993 May;28 Suppl 5:67-70. doi: 10.1007/BF02989209.
The immunoglobulin M antibody against hepatitis C viral capsid (core) antigen has been found to be a possible marker for acute infection in post-transfusion hepatitis C. We extended this observation to a sporadic hepatitis C. In addition, a modified recombinant immunoblot assay (RIBA) that contained three HCV antigens (capsid, NS3 and NS5) in one strip was developed and used to study the antibody response among patients. This allowed a simultaneous comparison of either immunoglobulin M or G specific to individual HCV antigen and yielded more information. The assay was found useful in the diagnosis of acute hepatitis C in clinically uncertain cases.
抗丙型肝炎病毒衣壳（核心）抗原的免疫球蛋白M抗体已被发现可能是输血后丙型肝炎急性感染的标志物。我们将这一观察结果扩展到散发性丙型肝炎。此外，还开发了一种改良的重组免疫印迹分析（RIBA），该分析在一条带上包含三种丙型肝炎病毒抗原（衣壳、NS3和NS5），并用于研究患者的抗体反应。这使得能够同时比较针对单个丙型肝炎病毒抗原的免疫球蛋白M或G，并获得更多信息。该分析方法在临床诊断不明确的急性丙型肝炎病例中很有用。
