Kim C, Tse H Y
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
J Neuroimmunol. 1993 Jul;46(1-2):129-36. doi: 10.1016/0165-5728(93)90242-q.
For the purpose of tracking the migration of donor T cells in chronic relapsing EAE (CREAE), a Thy-1 congenic mouse strain on the SJL genetic background was generated. T cells from these distinguished from those of SJL (Thy-1.2+) by staining with specific anti-Thy-1 antibodies. In adoptive EAE, SJL.Thy-1a mice exhibited similar chronic remitting and relapsing cycles as in SJL mice. Reciprocal transfer of adoptive EAE had also been successfully carried out between the two Thy-1 disparate strains. The lack of alloreactivity against the Thy-1 antigen was further demonstrated by the absence of reactivity in the vitro mixed lymphocyte cultures and in vivo skin graft rejection between the two strains. The establishment of the SJL.Thy-1a mouse strain should prove to be a valuable tool for further dissecting the complex process of relapsing EAE.
为了追踪供体T细胞在慢性复发性实验性自身免疫性脑脊髓炎（CREAE）中的迁移情况，构建了一种SJL遗传背景下的Thy-1同源小鼠品系。通过用特异性抗Thy-1抗体染色，这些小鼠的T细胞与SJL（Thy-1.2+）的T细胞区分开来。在过继性EAE中，SJL.Thy-1a小鼠表现出与SJL小鼠相似的慢性缓解和复发周期。两种Thy-1不同的品系之间也成功进行了过继性EAE的相互转移。体外混合淋巴细胞培养以及两种品系之间的体内皮肤移植排斥反应均无反应性，进一步证明了对Thy-1抗原缺乏同种异体反应性。SJL.Thy-1a小鼠品系的建立应被证明是进一步剖析复发性EAE复杂过程的有价值工具。
