可溶性脑一氧化氮合酶与NADPH-黄递酶活性之间的相关性仅在组织暴露于固定剂后才可见。
A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative.
作者信息
Matsumoto T, Nakane M, Pollock J S, Kuk J E, Förstermann U
机构信息
Department of Pharmacology, Northwestern University Medical School, Chicago, IL 60611.
出版信息
Neurosci Lett. 1993 May 28;155(1):61-4. doi: 10.1016/0304-3940(93)90673-9.
In histochemical studies using fixed brain tissue, NADPH-diaphorase has been found to be colocalized with soluble nitric oxide synthase. In the present study, using fresh tissues from eight different regions of rat brain, NADPH-diaphorase activity was found mostly in the particulate fraction, whereas most of the nitric oxide synthase activity was located to the cytosolic fraction. Also, the distribution of NADPH-diaphorase activity among brain regions was different from that of nitric oxide synthase. Pretreatment of the fractions with paraformaldehyde virtually abolished the NADPH-diaphorase activity in the particulate fraction, whereas 40-60% of the NADPH-diaphorase activity remained intact in the cytosolic fraction. These results suggest that during fixation most NADPH-diaphorase activity is inactivated and only some of the NADPH-diaphorase activity associated with soluble nitric oxide synthase remains intact.
在利用固定脑组织进行的组织化学研究中，已发现还原型辅酶Ⅱ黄递酶与可溶性一氧化氮合酶共定位。在本研究中，使用来自大鼠脑八个不同区域的新鲜组织，发现还原型辅酶Ⅱ黄递酶活性大多存在于微粒部分，而大部分一氧化氮合酶活性位于胞质部分。此外，还原型辅酶Ⅱ黄递酶活性在脑区之间的分布与一氧化氮合酶的分布不同。用多聚甲醛预处理这些部分实际上消除了微粒部分的还原型辅酶Ⅱ黄递酶活性，而胞质部分中40%-60%的还原型辅酶Ⅱ黄递酶活性保持完整。这些结果表明，在固定过程中，大多数还原型辅酶Ⅱ黄递酶活性被灭活，只有一些与可溶性一氧化氮合酶相关的还原型辅酶Ⅱ黄递酶活性保持完整。
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