Fujio Y, Yamada F, Takahashi K, Shibata N
Center for Adult Diseases, Osaka, Japan.
Biochem Biophys Res Commun. 1993 Aug 31;195(1):79-83. doi: 10.1006/bbrc.1993.2012.
Platelet-derived growth factor (PDGF) plays important roles in proliferation of smooth muscle cells (SMCs). PDGF is also known to induce the cytoskeletal reorganization as a chemoattractant. In this communication, we demonstrate that the tyrosine kinase inhibitor herbimycin-A (HM-A) inhibited the effects of PDGF on SMCs in proliferation, phenotypic modulation, and reorganization of F-actin. Another tyrosine kinase inhibitor genistein also inhibited reorganization of F-actin. Since both tyrosine kinase inhibitors reduced PDGF-dependent tyrosine phosphorylation, tyrosine kinase activities may be involved not only in proliferation but also in reorganization of F-actin.
血小板衍生生长因子（PDGF）在平滑肌细胞（SMC）增殖中发挥重要作用。PDGF作为一种趋化因子，还可诱导细胞骨架重组。在本报告中，我们证明酪氨酸激酶抑制剂赫比霉素-A（HM-A）可抑制PDGF对SMC在增殖、表型调节和F-肌动蛋白重组方面的作用。另一种酪氨酸激酶抑制剂染料木黄酮也可抑制F-肌动蛋白的重组。由于这两种酪氨酸激酶抑制剂均降低了PDGF依赖性酪氨酸磷酸化，酪氨酸激酶活性可能不仅参与增殖，还参与F-肌动蛋白的重组。
