Fujio Y, Yamada F, Takahashi K, Shibata N
Center for Adult Diseases, Osaka, Japan.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):997-1002. doi: 10.1006/bbrc.1993.2348.
PDGF promotes growth of smooth muscle cells (SMCs) and induces morphological changes. We investigated the effects of PDGF on cell-matrix adhesion of SMCs. PDGF reorganized integrin distribution as well as actin filament structure. PDGF-treated cells attached more loosely to fibronectin than non-treated cells. Decreased expression of alpha-actin by PDGF was antagonized by coating culture dishes with fibronectin but not with vitronectin and laminin. The inhibition of cell adhesion by GRGDSP oligopeptide and anti-fibronectin receptor antibody led to the reduction in alpha-actin expression. These results suggest that PDGF may modulate phenotype of SMCs by altering fibronectin-integrin interaction.
血小板衍生生长因子（PDGF）可促进平滑肌细胞（SMC）的生长并诱导其形态变化。我们研究了PDGF对SMC细胞与基质黏附的影响。PDGF重组了整合素分布以及肌动蛋白丝结构。与未处理的细胞相比，经PDGF处理的细胞与纤连蛋白的附着更为松散。用纤连蛋白而非玻连蛋白和层粘连蛋白包被培养皿可拮抗PDGF诱导的α-肌动蛋白表达降低。GRGDSP寡肽和抗纤连蛋白受体抗体对细胞黏附的抑制导致α-肌动蛋白表达减少。这些结果表明，PDGF可能通过改变纤连蛋白-整合素相互作用来调节SMC的表型。
