信号系统中的受体相互作用：趋化反应中核糖受体与半乳糖受体之间的竞争。
Receptor interactions in a signalling system: competition between ribose receptor and galactose receptor in the chemotaxis response.
作者信息
Strange P G, Koshland D E
出版信息
Proc Natl Acad Sci U S A. 1976 Mar;73(3):762-6. doi: 10.1073/pnas.73.3.762.
Abstract
Galactose and ribose receptors are shown to compete for a common site by comparing the specificities of the purified receptors with the specificity of the whole organism response. Ribose does not bind to the purified galactose receptor but inhibits the in vivo galactose response. A mutant lacking the ribose receptor shows no inhibition of the galactose response even at very high ribose concentrations. The results can be explained by a mechanism involving (a) a ligand-induced conformational change in the receptor which produces a combining site with affinity for the molecule that serves as a first component of the signalling system and (b) a limited number of such first component molecules that cannot bind both receptors simultaneously. The mechanism may have considerable generality for sensory, hormonal, and transport systems.
摘要
通过比较纯化受体的特异性与整个生物体反应的特异性，发现半乳糖和核糖受体竞争一个共同位点。核糖不与纯化的半乳糖受体结合，但会抑制体内半乳糖反应。缺乏核糖受体的突变体即使在非常高的核糖浓度下也不会抑制半乳糖反应。这些结果可以通过一种机制来解释，该机制涉及：(a) 配体诱导受体的构象变化，产生对作为信号系统第一成分的分子具有亲和力的结合位点；(b) 数量有限的此类第一成分分子不能同时结合两种受体。该机制可能在感觉、激素和运输系统中具有相当的普遍性。
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