包含亨廷顿舞蹈症基因区域的转录图谱。

A transcription map of the region containing the Huntington disease gene.

作者信息

Rommens J M, Lin B, Hutchinson G B, Andrew S E, Goldberg Y P, Glaves M L, Graham R, Lai V, McArthur J, Nasir J

机构信息

Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.

出版信息

Hum Mol Genet. 1993 Jul;2(7):901-7. doi: 10.1093/hmg/2.7.901.

DOI:10.1093/hmg/2.7.901

PMID:7689900

Abstract

A transcription map of the Huntington disease gene region was generated by a direct cDNA selection strategy using genomic DNA from the 4p16.3 region surrounding the D4S95 and D4S127 loci. A total of 58 cDNA fragments were obtained from cDNAs derived from fetal brain, frontal cortex, liver and bone marrow following hybridization to overlapping YACs from this region. These cDNA clones were aligned into transcription units by hybridization to specific mRNAs, by sequence overlap and by physical mapping onto overlapping YAC clones. Nine separate transcription units spanning approximately one megabase were detected by RNA hybridization. They represent a minimum number of genes in this region and do not include those genes expressed specifically in tissues not used for the hybridization. The transcription map that is provided by the cDNA segments will lead to the generation of a detailed gene map of this region.

摘要

利用来自围绕D4S95和D4S127基因座的4p16.3区域的基因组DNA，通过直接cDNA选择策略构建了亨廷顿病基因区域的转录图谱。在与该区域重叠酵母人工染色体（YAC）杂交后，从胎儿脑、额叶皮质、肝脏和骨髓来源的cDNA中总共获得了58个cDNA片段。通过与特定mRNA杂交、序列重叠以及在重叠YAC克隆上进行物理定位，将这些cDNA克隆排列成转录单位。通过RNA杂交检测到9个跨越约1兆碱基的独立转录单位。它们代表了该区域的最少基因数量，不包括那些在未用于杂交的组织中特异性表达的基因。由cDNA片段提供的转录图谱将有助于生成该区域的详细基因图谱。