Hadano S, Ishida Y, Tomiyasu H, Yamamoto K, Bates G P, Ikeda J E
NeuroGenes, International Research Exchange Project, Research Development Corporation of Japan (JRDC), Tokai University School of Medicine, Kanagawa, Japan.
DNA Res. 1996 Aug 31;3(4):239-55. doi: 10.1093/dnares/3.4.239.
Six hundred and twenty-seven cDNA clones from human brain cDNA libraries were characterized and integrated into a transcript map of the 1-Mb region on human chromosome 4p16.3 containing the Huntington's disease (HD) gene. Six hundred and seventy-two cDNA clones were obtained by a direct screening of the cDNA libraries, probing with pools of single copy microclones generated from the HD region specific yeast artificial chromosome (YAC)-DNA. So far, 93% of the obtained clones (627 cDNA clones) have been mapped onto the 1-Mb HD gene region by hybridization with HD region-specific cosmid, P1 and YAC clones. DNA sequence and expression analyses revealed that several cDNA clones might encode novel genes, some of which are situated within or close to the IT15, IT11, and alpha-adducin (ADD1) gene region, suggesting the presence of the overlapping genes in this region. This collection of cDNA clones will greatly facilitate the construction of the complete map of the transcripts in the HD region.
对来自人脑cDNA文库的627个cDNA克隆进行了表征，并将其整合到人类4号染色体p16.3区域1 Mb范围内包含亨廷顿舞蹈病（HD）基因的转录图谱中。通过直接筛选cDNA文库获得了672个cDNA克隆，用从HD区域特异性酵母人工染色体（YAC）-DNA产生的单拷贝微克隆池进行探测。到目前为止，通过与HD区域特异性黏粒、P1和YAC克隆杂交，已将93%的获得克隆（627个cDNA克隆）定位到1 Mb的HD基因区域。DNA序列和表达分析表明，几个cDNA克隆可能编码新基因，其中一些位于IT15、IT11和α-内收蛋白（ADD1）基因区域内或附近，提示该区域存在重叠基因。这组cDNA克隆将极大地促进HD区域转录本完整图谱的构建。
