Ando M, Dannenberg A M, Courtade E, Shima K
Proc Soc Exp Biol Med. 1976 Mar;151(3):491-4. doi: 10.3181/00379727-151-39242.
Rabbits were infected or reinfected intradermally in multiple sites with BCG. One day before the BCG infection they were injected iv with a single pulse of tritiated thymidine (3HT). At various times thereafter, the BCG lesions were biopsied and evaluated for 3HT-labeled mononuclear cells (MN), which are mostly macrophages but include some lymphocytes. Periodically Old Tuberculin (OT) was infected intradermally, creating MN traps, which were biopsied and evaluated 1 day after their onset. These traps monitor the population of 3HT-labeled MN in the blood stream that readily enters sites of inflammation. During the first 5 days after the 3HT pulse, a higher percentage of labeled MN accumulated in tuberculin traps on rabbits reinfected with BCG than in traps on rabbits with primary infection. The high percentage of labeled MN in the former was probably due to the more intense inflammation cuased by preexisting tuberculin hypersensitivity. By 8 days after the 3HT pulse, a lower percentage of labeled MN had accumulated in BCG lesions of reinfection than in primary BCG lesions. Evidently the presence of delayed hypersensitivity increased the turnover of MN in tuberculous lesions. By 15 days after the 3HT pulse, the percentage of 3HT-labeled MN was much higher in BCG lesions than in 1-day traps, suggesting that such MN had accumulated in the lesions over a period of several days.
用卡介苗对兔子进行多个部位的皮内感染或再感染。在卡介苗感染前一天，给它们静脉注射一次氚标记胸腺嘧啶核苷（3HT）。此后在不同时间，对卡介苗病灶进行活检，并评估3HT标记的单核细胞（MN），这些细胞大多是巨噬细胞，但也包括一些淋巴细胞。定期皮内注射旧结核菌素（OT），形成MN陷阱，在其出现后1天进行活检和评估。这些陷阱监测血流中易于进入炎症部位的3HT标记MN的数量。在3HT脉冲后的前5天，再次感染卡介苗的兔子的结核菌素陷阱中积累的标记MN百分比高于初次感染兔子的陷阱。前者标记MN的高百分比可能是由于先前存在的结核菌素超敏反应引起的更强烈炎症。到3HT脉冲后8天，再感染的卡介苗病灶中积累的标记MN百分比低于初次卡介苗病灶。显然，迟发型超敏反应的存在增加了结核病灶中MN的周转率。到3HT脉冲后15天，卡介苗病灶中3HT标记MN的百分比远高于1天陷阱中的百分比，这表明此类MN在几天内积累在病灶中。
