Tsuda T, Dannenberg A M, Ando M, Abbey H, Corrin A R
Am J Pathol. 1976 May;83(2):255-68.
Rabbits were injected intradermally in multiple sites with BCG. Four days after the BCG injection, they were injected intravenously with a single pulse of tritiated thymidine (3H-TdR) at various times thereafter, the BCG lesions were biopsied and evaluated for 3H-TdR-labeled mononuclear cells (MN). Periodically, Old Tuberculin (OT) was injected intradermally, creating MN traps which were biopsied and evaluated 1 day after their onset. 3H-TdR-labeled cells were also evaluated in samples of blood. During the first 8 days after the 3H-TdR pulse, the labeled MNs represent short-lived cells, i.e., recently dividing monocytes and lymphocytes. During this time, the percentage of labeled MNs in the blood, in the traps, and in the BCG lesions rose and fell together. This result suggests that the majority of the MNs in the BCG lesions had a turnover rate of about a week. By 12 days and afterward, the percentage of MNs labeled by 3H-TdR in the blood was higher than that in the BCG lesions which was in turn higher than that in the traps. At this time the circulating MN population probably contained labeled long-lived lymphocytes that did not enter inflammatory sites (the traps) as readily as the short-lived lymphocytes. The labeled MNs remaining in the BCG lesions probably did not divide and dilute out their 3H-TdR label as readily as those that were trapped from the bone marrow via the blood. The percentage of MNs labeled with 3H-TdR in the traps had decreased to about one-fifteenth of its peak value by 12 days, suggesting that the bone marrow's supply of labeled MNs was depleted at this time, except for the few cells labeled as a result of 3H-TdR reutilization.
将卡介苗在多个部位皮内注射到兔子体内。卡介苗注射4天后，在之后的不同时间给兔子静脉注射单次脉冲的氚标记胸腺嘧啶核苷（³H-TdR），然后对卡介苗损伤部位进行活检，并评估³H-TdR标记的单核细胞（MN）。定期皮内注射旧结核菌素（OT），形成MN陷阱，在其出现后1天进行活检和评估。还对血液样本中的³H-TdR标记细胞进行评估。在³H-TdR脉冲后的前8天，标记的MN代表寿命较短的细胞，即最近分裂的单核细胞和淋巴细胞。在此期间，血液、陷阱和卡介苗损伤部位中标记的MN百分比一起上升和下降。这一结果表明，卡介苗损伤部位的大多数MN周转率约为一周。到12天及之后，血液中被³H-TdR标记的MN百分比高于卡介苗损伤部位，而卡介苗损伤部位又高于陷阱中的。此时，循环中的MN群体可能包含标记的长寿淋巴细胞，它们不像短命淋巴细胞那样容易进入炎症部位（陷阱）。留在卡介苗损伤部位的标记MN可能不像那些通过血液从骨髓中捕获的MN那样容易分裂并稀释其³H-TdR标记。到12天时，陷阱中被³H-TdR标记的MN百分比已降至其峰值的约十五分之一，这表明此时骨髓中标记MN的供应已耗尽，除了少数因³H-TdR再利用而标记的细胞。
