Wake K, Sato T
Department of Anatomy, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Cell Tissue Res. 1993 Aug;273(2):227-37. doi: 10.1007/BF00312824.
The aim of the present investigation was to elucidate the intralobular heterogeneity of the perisinusoidal stellate cells (fat-storing cells, lipocytes) in the porcine liver. Their three-dimensional structure, desmin immunoreactivity and vitamin-A storage were studied by use of the Golgi silver, immunocytochemical and gold chloride methods. In order to locate the stellate cells, the hepatic lobules were divided into 10 zones. The stellate cells were readily identified in Golgi preparations by their striking dendritic appearance with branching processes encompassing the sinusoids. The stellate cells in the centrolobular zones were conspicuously dendritic with longer processes in comparison to those emitted by periportal elements. Such arborizations were studded with numerous thorn-like microprojections. Desmin immunoreaction in the periportal zones was stronger than that in the centrolobular zones. Vitamin-A storage in the stellate cells was well developed in zones 2-4, but reduced gradually toward the central region. The perisinusoidal stellate cells display marked heterogeneity in morphology and function based on their zonal location in the hepatic lobule.
本研究的目的是阐明猪肝内窦周星状细胞（贮脂细胞、脂肪细胞）的小叶内异质性。通过高尔基银染法、免疫细胞化学法和氯化金法研究了它们的三维结构、结蛋白免疫反应性和维生素A储存情况。为了定位星状细胞，将肝小叶分为10个区。在高尔基染色标本中，星状细胞很容易通过其显著的树突状外观被识别，其分支过程围绕着血窦。与门周区的星状细胞相比，中央小叶区的星状细胞呈明显的树突状，且有更长的突起。这种分支上布满了许多刺状微突起。门周区的结蛋白免疫反应比中央小叶区更强。星状细胞中的维生素A储存在2-4区发育良好，但向中央区域逐渐减少。基于其在肝小叶中的区域位置，窦周星状细胞在形态和功能上表现出明显的异质性。
