Matsukura H, Butkowski R J, Fish A J
Department of Laboratory Medicine, University of Minnesota, Minneapolis 55455.
Nephron. 1993;64(4):532-9. doi: 10.1159/000187396.
To determine the nature of Goodpasture antibody-reactive epitopes on the globular domains of collagen IV [non collagenous (NC) domain], Goodpasture antigen was characterized by immunochemical and immunohistological techniques using affinity-purified (AP) Goodpasture autoantibodies. Affinity purification of Goodpasture autoantibodies toward alpha 1(IV), alpha 2(IV), and alpha 3(IV) NC domains was performed and revealed antigenic cross-reactivity with all alpha(IV) NC domain subunits, which was confirmed by inhibition ELISA. Since by immunofluorescent microscopy all 3 AP Goodpasture antibodies only stained the central portion (lamina densa) of bovine glomerular basement membrane, it appears that there are common epitopes reactive with Goodpasture autoantibodies present on alpha 1(IV) NC, alpha 2(IV) NC, and alpha 3(IV) NC domains.
为了确定IV型胶原球状结构域[非胶原（NC）结构域]上Goodpasture抗体反应性表位的性质，使用亲和纯化（AP）的Goodpasture自身抗体，通过免疫化学和免疫组织学技术对Goodpasture抗原进行了表征。对针对α1（IV）、α2（IV）和α3（IV）NC结构域的Goodpasture自身抗体进行了亲和纯化，结果显示与所有α（IV）NC结构域亚基存在抗原交叉反应性，这通过抑制ELISA得到了证实。由于通过免疫荧光显微镜观察，所有3种AP Goodpasture抗体仅对牛肾小球基底膜的中央部分（致密层）进行染色，因此看来在α1（IV）NC、α2（IV）NC和α3（IV）NC结构域上存在与Goodpasture自身抗体反应的共同表位。
