Vafa A Z, Grover P K, Pretlow T G, Resnick M I
Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio.
Urology. 1993 Aug;42(2):138-43. doi: 10.1016/0090-4295(93)90636-o.
Our previous studies demonstrated that extracts of the cancerous prostate tissues exhibit increased activities of arginase and hexosaminidase and decreased activity of leucine aminopeptidase. In an endeavor to devise a reliable, noninvasive and cost effective method for the diagnosis of prostate carcinoma, prostate fluids collected from 123 patients were analyzed for arginase, hexosaminidase, and leucine aminopeptidase. Based on the clinical diagnoses, the patients were divided into four groups: 13 men with chronic prostatitis, 29 with no recognized prostatic lesions, 63 with benign prostatic hyperplasia, and 18 with prostate cancer. The prostate fluids had no detectable arginase activity. The means of the total (units/mL) and the specific activities (units/mg of protein) of hexosaminidase and leucine aminopeptidase varied, albeit there were no significant differences in the four groups of patients recruited in this study. It was concluded that the profiles of enzymes in prostate fluids might differ from that in the secreting prostate tissues and that the results of enzyme analysis in prostate fluids for the diagnosis of cancer should be interpreted with caution.
我们之前的研究表明，癌性前列腺组织提取物中精氨酸酶和己糖胺酶的活性增加，而亮氨酸氨肽酶的活性降低。为了设计一种可靠、无创且经济高效的前列腺癌诊断方法，我们对123例患者采集的前列腺液进行了精氨酸酶、己糖胺酶和亮氨酸氨肽酶分析。根据临床诊断，患者被分为四组：13例患有慢性前列腺炎，29例无明显前列腺病变，63例患有良性前列腺增生，18例患有前列腺癌。前列腺液中未检测到精氨酸酶活性。己糖胺酶和亮氨酸氨肽酶的总活性（单位/毫升）和比活性（单位/毫克蛋白质）均值有所不同，尽管在本研究纳入的四组患者中没有显著差异。研究得出结论，前列腺液中的酶谱可能与分泌前列腺组织中的酶谱不同，并且在使用前列腺液酶分析结果诊断癌症时应谨慎解读。
