Lei Z M, Toth P, Rao C V, Pridham D
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Kentucky 40292.
J Clin Endocrinol Metab. 1993 Sep;77(3):863-72. doi: 10.1210/jcem.77.3.7690366.
The human uterus, including its blood vessels, contains hCG/human LH receptors. We now demonstrate that human fallopian tubes also contain a 4.4-kilobase hCG/LH receptor mRNA transcript and an 80-kilodalton immunoreactive protein that can bind [125I]hCG. Tubal mucosa contain more receptor transcripts, receptor protein, and [125I] hCG binding than the tubal smooth muscle or blood vessels. Human fallopian tubes also contain hCG protein and a 0.6-kilobase hCG alpha mRNA transcript. However, very little hCG is found in tubal cell layers other than mucosa. Ampullary segments contain more hCG/LH receptors and hCG than isthmus. Secretory phase tubes contain more than proliferative phase, postpartum, or postmenopause tubes. Incubation with highly purified hCG resulted in an increase in catalytically active 5-lipoxygenase, cyclooxygenase-1, and cyclooxygenase-2 enzymes in tubal tissues. In summary, human fallopian tubes, which have never previously been considered a direct target of hCG/LH action, express functional hCG/LH receptor gene as well as the gene of its ligand. These novel findings suggest numerous possibilities of both physiological and pathological importance in human fallopian tubes.
人类子宫，包括其血管，含有绒毛膜促性腺激素/人促黄体生成素（hCG/human LH）受体。我们现在证明，人类输卵管也含有一种4.4千碱基的hCG/LH受体信使核糖核酸转录本和一种能结合[125I]hCG的80千道尔顿免疫反应蛋白。输卵管黏膜比输卵管平滑肌或血管含有更多的受体转录本、受体蛋白和[125I]hCG结合。人类输卵管还含有hCG蛋白和一种0.6千碱基的hCGα信使核糖核酸转录本。然而，除黏膜外，在输卵管细胞层中发现的hCG很少。壶腹部段比峡部含有更多的hCG/LH受体和hCG。分泌期输卵管比增殖期、产后或绝经后输卵管含有更多。用高度纯化的hCG孵育导致输卵管组织中催化活性5-脂氧合酶、环氧化酶-1和环氧化酶-2酶增加。总之，人类输卵管以前从未被认为是hCG/LH作用的直接靶点，它表达功能性hCG/LH受体基因及其配体基因。这些新发现提示了人类输卵管在生理和病理方面具有许多重要的可能性。
