Hagen L S, Jacquemond M, Lepingle A, Lot H, Tepfer M
Station de Pathologie Végétale, INRA, Montfavet, France.
Virology. 1993 Oct;196(2):619-28. doi: 10.1006/viro.1993.1518.
Cacao swollen shoot virus is classified as a badnavirus based on its nonenveloped, bacilliform particle morphology and double-stranded DNA genome. A complete copy of the genome was cloned into a plasmid vector and the sequence was determined from 75 overlapping subclones covering both strands. The genome contains 7161 base pairs and possesses an intergenic region and five putative open reading frames (ORF) capable of coding for proteins > 10 kDa. All of the ORFs are present on the plus-strand. ORF 1 (17 kDa) and ORF 2 (14 kDa) encode proteins of unknown function. The large ORF 3 (211 kDa) encodes a polyprotein that can be divided into three regions. Based on distant homologies with viral movement proteins, region 1 may encode a protein involved in cell-to-cell spread, while region 2 encodes the viral capsid protein. Region 3 contains consensus sequences for viral aspartyl proteinase, reverse transcriptase, and ribonuclease H characteristic of pararetroviruses. The last two ORFs (13 and 14 kDa) overlap ORF 3 and are not present in the other badnaviruses described.
可可肿枝病毒根据其无包膜的杆状颗粒形态和双链DNA基因组被归类为杆状DNA病毒。基因组的完整拷贝被克隆到质粒载体中，并从覆盖两条链的75个重叠亚克隆中确定了序列。该基因组包含7161个碱基对，拥有一个基因间隔区和五个推定的开放阅读框（ORF），能够编码大于10 kDa的蛋白质。所有的开放阅读框都位于正链上。开放阅读框1（17 kDa）和开放阅读框2（14 kDa）编码功能未知的蛋白质。大的开放阅读框3（211 kDa）编码一种多蛋白，可分为三个区域。基于与病毒运动蛋白的远缘同源性，区域1可能编码一种参与细胞间传播的蛋白质，而区域2编码病毒衣壳蛋白。区域3包含类逆转录病毒的病毒天冬氨酸蛋白酶、逆转录酶和核糖核酸酶H的共有序列。最后两个开放阅读框（13和14 kDa）与开放阅读框3重叠，在所描述的其他杆状DNA病毒中不存在。
