Wei L L, Leach M W, Miner R S, Demers L M
Department of Medicine, Pennsylvania State University, Milton S. Hershey Medical Center, Hershey 17033.
Biochem Biophys Res Commun. 1993 Sep 15;195(2):525-32. doi: 10.1006/bbrc.1993.2077.
We report on the presence of progesterone receptor and its mRNA in a human osteoblast-like cell line (HOS TE85) using recombinant DNA methods, saturation analysis, specific immunoprecipitation with an anti-progesterone receptor antibody, and Northern blot analysis. These osteoblast-like cells were also found to be responsive to progesterone in a dose-dependent fashion through an augmentation in cellular alkaline phosphatase activity. These results support the presence of progesterone receptors in bone cells and suggest a direct effort of progesterone and/or antiprogestins on osteoblast cell function.
我们运用重组DNA方法、饱和分析、抗孕激素受体抗体特异性免疫沉淀以及Northern印迹分析，报告了人成骨细胞样细胞系（HOS TE85）中孕激素受体及其mRNA的存在情况。这些成骨细胞样细胞还被发现通过细胞碱性磷酸酶活性的增强，以剂量依赖方式对孕激素产生反应。这些结果支持骨细胞中存在孕激素受体，并提示孕激素和/或抗孕激素对成骨细胞功能有直接作用。
