Takano K, Malamud D, Bennick A, Oppenheim F, Hand A R
Department of Oral Histology, Nagasaki University School of Dentistry, Japan.
Crit Rev Oral Biol Med. 1993;4(3-4):399-405. doi: 10.1177/10454411930040032001.
Electron microscopic immunocytochemistry was used to investigate the distribution of four secretory proteins within the granules of human parotid and submandibular gland acinar cells. The results demonstrate that the distribution of the individual proteins differs significantly. Amylase is concentrated within the electron-dense regions of the granules, whereas agglutinin is present mainly in the regions of lower density. In contrast, the proline-rich proteins and histatins are distributed throughout the content of the granules and do not appear to be concentrated in either the high- or low-density regions. The results also suggest that different mechanisms, such as self-aggregation or ionic complexes with metal ions and charged macromolecules, may be involved in the packaging of individual proteins within the same granule. Further, they also show that among the salivary glands of various species, the intragranular distribution of similar proteins (e.g., amylase and proline-rich proteins) may differ.
采用电子显微镜免疫细胞化学方法研究了四种分泌蛋白在人腮腺和颌下腺腺泡细胞颗粒内的分布情况。结果表明，各蛋白的分布存在显著差异。淀粉酶集中在颗粒的电子致密区域，而凝集素主要存在于低密度区域。相比之下，富含脯氨酸的蛋白质和组蛋白分布于颗粒的整个内容物中，似乎并不集中在高密度或低密度区域。结果还表明，不同的机制，如自我聚集或与金属离子和带电大分子形成离子复合物，可能参与了同一颗粒内单个蛋白质的包装。此外，研究结果还表明，在不同物种的唾液腺中，类似蛋白质（如淀粉酶和富含脯氨酸的蛋白质）的颗粒内分布可能有所不同。
