Malaĭtsev V V, Azhipa O Ia
Biull Eksp Biol Med. 1993 Jun;115(6):645-6.
The model of T-dependent dopamine-protein conjugates prepared by the glutaraldehyde method was used to study the influence of hapten valence and molecular weight on the immunogenicity of these conjugates. The immunogenicity of dopamine-protein conjugates increased in the range of DA4-BSA--DA5,8-BSA--DA14-BSA and decreased with further increase of hapten density. It was assumed that partial tolerogenic properties of DA18-22-BSA were due to nonspecific mechanisms associated with changes in the physical molecular characteristics of the antigen during the procedure of conjugation, rather than to specific mechanisms. The latter are more typical for low substituted conjugates.
采用戊二醛法制备的T细胞依赖性多巴胺-蛋白质缀合物模型，用于研究半抗原价数和分子量对这些缀合物免疫原性的影响。多巴胺-蛋白质缀合物的免疫原性在DA4-BSA - DA5,8-BSA - DA14-BSA范围内增加，并随着半抗原密度的进一步增加而降低。推测DA18-22-BSA的部分致耐受性特性是由于在缀合过程中与抗原物理分子特性变化相关的非特异性机制，而非特异性机制。后者对于低取代缀合物更为典型。
