Avtalion R R, Milgrom L
Immunology. 1976 Oct;31(4):589-94.
Sodium penicillin was conjugated to sheep erythrocytes and optimal quantities, added to a 5% SRBC suspension, were determined for haemagglutination (12-5 mg/ml) and for haemolysis (50 mg/ml) using carp antibodies and carp complement. The epitope density on the BSA molecule was gradually increased, when increasing amounts of sodium-penicillin were added to a constant quantity of BSA, until a maximum of about thirty penicilloyl groups were bound. Low conjugates, having less than seven haptenic groups per one BSA molecule, were found to stimulate carp for both anti-hapten and anti-carrier antibodies. The higher conjugates having seven and more haptenic groups were found to stimulate carp for anti-panicilloyl antibodies but not for anti-BSA antibodies. A booster dose with native BSA, given to the Pen30 BSA preimmunized carp, gave rise directly to a secondary-like response. In the rabbits, however, both heavy and low conjugates were found to stimulate antibody production for the hapten as well as for the carrier. It was suggested that the modified BSA in the heavy conjugates loses its ability to stimulate B cells, probably due to a decrease in local concentration of antigenic determinants in the BSA molecule, but its ability to stimulate helper cells is not affected for this reason.
将青霉素钠与绵羊红细胞结合，并使用鲤鱼抗体和鲤鱼补体，确定添加到5% SRBC悬液中的最佳量，用于血凝反应（12.5 mg/ml）和溶血反应（50 mg/ml）。当向恒定数量的牛血清白蛋白（BSA）中添加越来越多的青霉素钠时，BSA分子上的表位密度逐渐增加，直到最多约30个青霉素酰基团被结合。发现每一个BSA分子具有少于7个半抗原基团的低结合物能刺激鲤鱼产生抗半抗原和抗载体抗体。发现具有7个及更多半抗原基团的高结合物能刺激鲤鱼产生抗青霉素酰抗体，但不能刺激产生抗BSA抗体。给预先用Pen30 BSA免疫的鲤鱼注射一剂天然BSA，直接引发了类似二次免疫的反应。然而，在兔子中，发现重结合物和低结合物都能刺激产生针对半抗原和载体的抗体。有人提出，重结合物中修饰后的BSA失去了刺激B细胞的能力，这可能是由于BSA分子中抗原决定簇的局部浓度降低，但由于这个原因其刺激辅助细胞的能力并未受到影响。
