使用胶原凝胶基质对人泌尿生殖系统肿瘤进行体外化学敏感性试验。

In vitro chemosensitivity test for human genitourinary tumors using collagen gel matrix.

作者信息

Uchibayashi T, Egawa M, Hisazumi H, Hasegawa T, Tanaka T, Sasaki T

机构信息

Department of Urology, School of Medicine, Kanazawa University, Japan.

出版信息

Eur Urol. 1993;24(2):267-70. doi: 10.1159/000474307.

DOI:10.1159/000474307

PMID:7690707

Abstract

An in vitro chemosensitivity test using collagen gel matrix assay with quantitative evaluation of cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was applied to 40 human genitourinary tumor specimens. Chemosensitivity was determined in 34 (85%) of the 40 specimens. The anticancer drugs tested against 14 renal cell carcinomas showed lower activities than against 15 transitional cell carcinomas with different sensitivity patterns among the individual specimens demonstrated. Our assay system could be successfully applied to human genitourinary tumors and can be used as a practical and highly reproducible chemosensitivity test in vitro.

摘要

使用胶原凝胶基质分析并通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐对细胞活力进行定量评估的体外化学敏感性试验应用于40例人类泌尿生殖系统肿瘤标本。40例标本中有34例（85%）测定了化学敏感性。针对14例肾细胞癌测试的抗癌药物活性低于针对15例移行细胞癌的活性，且各标本间显示出不同的敏感性模式。我们的分析系统可成功应用于人类泌尿生殖系统肿瘤，并且可作为一种实用且高度可重复的体外化学敏感性试验。