唾液酸化路易斯x四糖的高效酶促合成。一种选择素型黏附分子的配体。
Efficient enzymatic synthesis of the sialyl-Lewisx tetrasaccharide. A ligand for selectin-type adhesion molecules.
作者信息
de Vries T, van den Eijnden D H, Schultz J, O'Neill R
机构信息
Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
FEBS Lett. 1993 Sep 20;330(3):243-8. doi: 10.1016/0014-5793(93)80881-t.
Sialyl-Lewisx (NeuAc alpha 2-->3Gal beta 1-->4[Fuc alpha 1-->3]GlcNAc] has been identified as a ligand for E-selectin, P-selectin and recently also for L-selectin. We have synthesized the sialyl-Lewisx tetrasaccharide by total enzymatic synthesis from N-acetyllactosamine using a placental alpha 2-->3-sialyltransferase specific for type-2 chain acceptors, followed by a cloned human alpha 1-->3-fucosyltransferase (FucTV, the 'plasma-type' enzyme). This procedure resulted in the tetrasaccharide in a 61% overall yield.
唾液酸化路易斯x（NeuAcα2→3Galβ1→4[Fucα1→3]GlcNAc）已被鉴定为E-选择素、P-选择素的配体，最近也被鉴定为L-选择素的配体。我们使用对2型链受体具有特异性的胎盘α2→3-唾液酸转移酶，通过从N-乙酰乳糖胺进行全酶促合成，随后使用克隆的人α1→3-岩藻糖基转移酶（FucTV，“血浆型”酶），合成了唾液酸化路易斯x四糖。该方法得到的四糖总产率为61%。
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