Koenig S, Woods R M, Brewah Y A, Newell A J, Jones G M, Boone E, Adelsberger J W, Baseler M W, Robinson S M, Jacobson S
Department of Immunology, MedImmune, Inc., Gaithersburg, MD 20878.
J Immunol. 1993 Oct 1;151(7):3874-83.
To understand the nature of the cytotoxic T cell response generated in human T lymphotropic virus type 1 (HTLV-1)-infected patients with HTLV-1-associated myelopathy/tropical spastic paraparesis, we cloned CTL from the peripheral blood and cerebrospinal fluid from patients with neurologic diseases and demonstrated the presence of HLA-A2, A3, and B14 restricted responses to the HTLV-1 p40x (tax) protein. We identified the minimal amino acid residues within the epitopes required for binding and recognition by HLA-A2- and B14-restricted CTL, identified the critical residues within the peptide sequence defining the HLA-A2-restricted response, and demonstrated that CTL can lyse T cells infected with HTLV-1. This study shows that the CTL response to HTLV-1 tax in patients with neurologic diseases is heterogenous in nature and is not confined to patients of a single HLA haplotype or to a specific region of the tax protein.
为了解人类嗜T淋巴细胞病毒1型（HTLV-1）感染的、患有HTLV-1相关脊髓病/热带痉挛性截瘫的患者体内产生的细胞毒性T细胞反应的本质，我们从患有神经疾病患者的外周血和脑脊液中克隆了细胞毒性T淋巴细胞（CTL），并证明存在对HTLV-1 p40x（tax）蛋白的HLA-A2、A3和B14限制性反应。我们确定了HLA-A2和B14限制性CTL结合和识别所需表位内的最小氨基酸残基，确定了定义HLA-A2限制性反应的肽序列内的关键残基，并证明CTL可裂解感染HTLV-1的T细胞。本研究表明，患有神经疾病的患者对HTLV-1 tax的CTL反应本质上是异质性的，并不局限于单一HLA单倍型的患者或tax蛋白的特定区域。
