Bieganowska K, Höllsberg P, Buckle G J, Lim D G, Greten T F, Schneck J, Altman J D, Jacobson S, Ledis S L, Hanchard B, Chin J, Morgan O, Roth P A, Hafler D A
Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
J Immunol. 1999 Feb 1;162(3):1765-71.
Human T cell lymphotropic virus-I (HTLV-I)-associated myelopathy is a slowly progressive neurologic disease characterized by inflammatory infiltrates in the central nervous system accompanied by clonal expansion of HTLV-I-reactive CD8+ T-cells. In patients carrying the HLA-A2 allele, the immune response is primarily directed to the Tax11-19 peptide. The frequency, activation state, and TCR usage of HLA-A2/Tax11-19 binding T cells in patients with HTLV-I-associated myelopathy was determined using MHC class I tetramers loaded with the Tax11-19 peptide. Circulating Tax11-19-reactive T cells were found at very high frequencies, approaching 1:10 circulating CD8+ T cells. T cells binding HLA-A2/Tax11-19 consisted of heterogeneous populations expressing different chemokine receptors and the IL-2R beta-chain but not the IL-2R alpha-chain. Additionally, Tax11-19-reactive CD8+ T cells used one predominant TCR Vbeta-chain for the recognition of the HLA-A2/Tax11-19 complex. These data provide direct evidence for high frequencies of circulating Tax11-19-reactive CD8+ T cells in patients with HTLV-I-associated myelopathy.
人类嗜T细胞病毒I型（HTLV-I）相关脊髓病是一种缓慢进展的神经系统疾病，其特征是中枢神经系统出现炎症浸润，并伴有HTLV-I反应性CD8 + T细胞的克隆性扩增。在携带HLA-A2等位基因的患者中，免疫反应主要针对Tax11-19肽。使用负载Tax11-19肽的MHC I类四聚体，确定了HTLV-I相关脊髓病患者中HLA-A2/Tax11-19结合T细胞的频率、激活状态和TCR使用情况。发现循环中Tax11-19反应性T细胞的频率非常高，接近每10个循环CD8 + T细胞中有1个。结合HLA-A2/Tax11-19的T细胞由表达不同趋化因子受体和IL-2Rβ链但不表达IL-2Rα链的异质群体组成。此外，Tax11-19反应性CD8 + T细胞使用一种主要的TCR Vβ链来识别HLA-A2/Tax11-19复合物。这些数据为HTLV-I相关脊髓病患者循环中Tax11-19反应性CD8 + T细胞的高频率提供了直接证据。
