从马尾分离的人周围神经系统髓磷脂的糖鞘脂。

Glycosphingolipids of human peripheral nervous system myelins isolated from cauda equina.

作者信息

Ogawa-Goto K, Ohta Y, Kubota K, Funamoto N, Abe T, Taki T, Nagashima K

机构信息

Department of Pediatrics, Teikyo University School of Medicine, Tokyo, Japan.

出版信息

J Neurochem. 1993 Oct;61(4):1398-403. doi: 10.1111/j.1471-4159.1993.tb13633.x.

DOI:10.1111/j.1471-4159.1993.tb13633.x

PMID:7690848

Abstract

Compositions of neutral and sulfated glucuronyl glycosphingolipids purified from human motor and sensory nerves and myelins were studied. Higher neutral glycosphingolipids (fraction B), which were separated from GalCer (fraction A), were analyzed by TLC and TLC-immunostaining. Both nerve myelins contained paragloboside (nLc4Cer) and nLc6Cer dominantly as major higher glycosphingolipids and very little globoside (Gb4Cer), whereas both nerves contained Gb4Cer and nLc4Cer. Besides these major glycosphingolipids, a neutral glycolipid containing asialoGM1 (Gg4Cer) epitope and other minor components such as ceramide trihexoside and ceramide dihexoside were detected in both nerves and their myelins. Furthermore, sulfated glucuronyl nLc4Cer and nLc6Cer, which were monoclonal antibody HNK-1 reactive glycolipids, were detected in both nerves and myelins.

摘要

对从人运动神经、感觉神经及髓鞘中纯化得到的中性和硫酸化葡糖醛酸糖鞘脂的组成进行了研究。从半乳糖神经酰胺（组分A）中分离出的高级中性糖鞘脂（组分B），通过薄层层析（TLC）和TLC免疫染色进行分析。两种神经髓鞘均主要含有副球蛋白三己糖神经酰胺（nLc4Cer）和nLc6Cer作为主要的高级糖鞘脂，而球蛋白三己糖神经酰胺（Gb4Cer）含量极少，而两种神经均含有Gb4Cer和nLc4Cer。除了这些主要的糖鞘脂外，在两种神经及其髓鞘中均检测到一种含有脱唾液酸GM1（Gg4Cer）表位的中性糖脂以及其他次要成分，如神经酰胺三己糖苷和神经酰胺二己糖苷。此外，在两种神经和髓鞘中均检测到硫酸化葡糖醛酸nLc4Cer和nLc6Cer，它们是单克隆抗体HNK-1反应性糖脂。