Rueff J, Brás A, Cristóvão L, Mexia J, Sá da Costa M, Pires V
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Portugal.
Mutat Res. 1993 Oct;289(2):197-204. doi: 10.1016/0027-5107(93)90070-v.
DNA strand breaks and chromosomal aberrations (CAs) were studied in human cells treated with hydrogen peroxide or with ionizing radiation. DNA strand breaks could be produced at dose levels of H2O2 much lower than those which induced CAs. Doses as low as 0.5 mM of H2O2 produced about as many DNA strand breaks as 2 Gy of 60Co gamma-radiation. On the other hand, as much as 20 mM H2O2 produced only half as many CAs as 1 Gy of 60Co gamma-radiation. The different mechanisms involved in the production of human genetic damage by H2O2 and gamma-radiation are discussed.
研究了用过氧化氢或电离辐射处理的人类细胞中的DNA链断裂和染色体畸变（CA）。产生DNA链断裂的过氧化氢剂量水平远低于诱导染色体畸变的剂量水平。低至0.5 mM的过氧化氢产生的DNA链断裂数量与2 Gy的60Coγ辐射产生的数量大致相同。另一方面，高达20 mM的过氧化氢产生的染色体畸变数量仅为1 Gy的60Coγ辐射产生数量的一半。讨论了过氧化氢和γ辐射产生人类遗传损伤所涉及的不同机制。
