Karawita Rohan, Senevirathne Mahinda, Athukorala Yasantha, Affan Abu, Lee Young-Jae, Kim Se-Kwon, Lee Joon-Baek, Jeon You-Jin
Faculty of Applied Marine Science, Cheju National University, Jeju 690-756, South Korea.
Mar Biotechnol (NY). 2007 Jul-Aug;9(4):479-90. doi: 10.1007/s10126-007-9007-3. Epub 2007 May 23.
The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for their antioxidant activity. Of the extracts tested, the AMG 300 L (an exo 1, 4-alpha-D-glucosidase) extract of P. duplex, the Viscozyme extract of Navicula sp., and the Celluclast extract of A. longipes provided the most potential as antioxidants. Meanwhile, the Termamyl extract of P. duplex in an H(2)O(2) scavenging assay exhibited an approximate 60% scavenging effect. In this study, we report that the DNA damage inhibitory effects of P. duplex (Termamyl extract) and D. fascicularis (Kojizyme extract) were nearly 80% and 69% respectively at a concentration of 100 microg/ml. Thus, it is suggested that the microalgae tested in this study yield promising DNA damage inhibitory properties on mouse lymphoma L 5178 cells that are treated with H(2)O(2). Therefore, microalgae such as P. duplex may be an excellent source of naturally occurring antioxidant compounds with potent DNA damage inhibition potential.
对从韩国济州岛三个栖息地（淡水、潮汐池和沿海底栖生物）采集的七种微藻（双盘盘星藻、束丝藻、紫菜嗜盐绿藻、单细胞奥尔特曼藻、长柄曲壳藻、舟形藻属和咖啡形双眉藻）的酶提取物进行了抗氧化活性研究。在所测试的提取物中，双盘盘星藻的AMG 300 L（一种外切1,4-α-D-葡萄糖苷酶）提取物、舟形藻属的Viscozyme提取物和长柄曲壳藻的Celluclast提取物作为抗氧化剂最具潜力。同时，双盘盘星藻的嗜热栖芽孢杆菌淀粉酶提取物在H₂O₂清除试验中表现出约60%的清除效果。在本研究中，我们报告了在浓度为100微克/毫升时，双盘盘星藻（嗜热栖芽孢杆菌淀粉酶提取物）和束丝藻（米曲霉糖化酶提取物）对DNA损伤的抑制作用分别接近80%和69%。因此，建议本研究中测试的微藻对用H₂O₂处理的小鼠淋巴瘤L 5178细胞具有有前景的DNA损伤抑制特性。所以，像双盘盘星藻这样的微藻可能是具有强大DNA损伤抑制潜力的天然抗氧化化合物的优秀来源。
