Förster E, Otten U, Frotscher M
Institute of Anatomy, University of Freiburg, FRG.
Neurosci Lett. 1993 Jun 11;155(2):216-9. doi: 10.1016/0304-3940(93)90711-s.
Using reverse transcription in combination with the polymerase chain reaction, the developmental expression of neurotrophins in organotypic slice cultures of rat hippocampus was investigated. Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNA levels after different time periods in vitro were compared with equivalent developmental stages in vivo. Our results show that neurotrophin expression occurs in hippocampal slice cultures with a similar time course as observed in the developing hippocampus in vivo. Thus, the development of neurotrophin expression in the hippocampus does not seem to be dependent on specific extrinsic afferents.
利用逆转录与聚合酶链反应相结合的方法，研究了神经营养因子在大鼠海马器官型切片培养物中的发育表达。将体外不同时间段后的神经生长因子（NGF）和脑源性神经营养因子（BDNF）mRNA水平与体内相应的发育阶段进行了比较。我们的结果表明，神经营养因子的表达在海马切片培养物中的时间进程与在体内发育中的海马中观察到的相似。因此，海马中神经营养因子表达的发育似乎不依赖于特定的外在传入神经。
