Moch H, Torhorst J, Dürmüller U, Feichter G E, Sauter G, Gudat F
Institute for Pathology, Basel University, Switzerland.
Pathol Res Pract. 1993 Jun;189(5):510-4. doi: 10.1016/S0344-0338(11)80357-2.
Tenascin is an extracellular matrix glycoprotein expressed during morphogenesis in embryonal life. It reappears in the stroma of benign and malignant tumors. The distribution of tenascin in variants of fibrocystic disease and infiltrating breast carcinoma was assessed in cryostat sections by immunofluorescence using a polyclonal antibody. The tenascin immunoreactivity was compared with various prognostic factors. In fibrocystic disease (n = 10), tenascin appeared as periductal and periacinar bands. In infiltrating carcinomas (n = 32) the tenascin expression was markedly increased. Tenascin immunoreactivity was noted around the ducts (78%), extended into the distal stroma (56%), or was distributed in smaller (reticular) septa around and within tumor-cell nests (34%). Nineteen percent of infiltrating carcinomas did not express tenascin. None of the patterns correlated with prognostic factors such as nodal metastasis, tumor necrosis, invasion of blood vessels, or with flow cytometry results, such as ploidy and S-phase fraction. However, a significantly higher reticular and periepithelial tenascin expression was noted in cases with increased stromal inflammatory reaction. These findings indicate that the appearance of tenascin is neither an indicator of malignancy nor predictive of invasiveness or metastasis but that it is related to local inflammatory response.
腱生蛋白是一种细胞外基质糖蛋白，在胚胎发育的形态发生过程中表达。它在良性和恶性肿瘤的基质中再次出现。使用多克隆抗体通过免疫荧光在低温恒温器切片中评估腱生蛋白在纤维囊性疾病和浸润性乳腺癌变体中的分布。将腱生蛋白免疫反应性与各种预后因素进行比较。在纤维囊性疾病（n = 10）中，腱生蛋白表现为导管周围和腺泡周围带。在浸润性癌（n = 32）中，腱生蛋白表达明显增加。在导管周围观察到腱生蛋白免疫反应性（78%），延伸至远端基质（56%），或分布在肿瘤细胞巢周围和内部较小的（网状）间隔中（34%）。19%的浸润性癌不表达腱生蛋白。这些模式均与预后因素如淋巴结转移、肿瘤坏死、血管侵犯或与流式细胞术结果如倍体和S期分数无关。然而，在基质炎症反应增加的病例中，观察到网状和上皮周围腱生蛋白表达明显更高。这些发现表明，腱生蛋白的出现既不是恶性肿瘤的指标，也不能预测侵袭性或转移，但它与局部炎症反应有关。
