Shoji T, Kamiya T, Tsubura A, Hatano T, Sakakura T, Yamamoto M, Morii S
Department of Pathology, Kansai Medical University, Osaka, Japan.
Virchows Arch A Pathol Anat Histopathol. 1992;421(1):53-6. doi: 10.1007/BF01607139.
Eighty-two cases of primary invasive breast carcinoma and adjacent "normal" mammary glands were examined immunohistochemically for tenascin expression and distribution. Formalin-fixed tissues pretreated with actinase were processed by the avidin-biotin complex method using anti-human tenascin monoclonal antibody (RBC1). In normal mammary glands, tenascin was distributed around the ducts and ductules but not around the acini. In carcinomas, a high incidence of tenascin-positive cases (greater than 67%) was seen with various histological appearances, with the exception of lobular carcinoma where a low incidence was found (25%). Although intense staining was seen around cancerous foci when compared with normal mammary glands, tenascin was often expressed at cancer-mesenchymal junctions with dense fibrotic stroma, but not at junctions with active inflammatory change and a loose fibrotic stroma. Tenascin, expression is not an all-or-none marker for mammary malignancy and the staining pattern suggests either a role in stimulating cancer cells or a host defence mechanism accompanied by a desmoplastic response to them.
对82例原发性浸润性乳腺癌及相邻的“正常”乳腺组织进行了免疫组织化学检查，以检测腱生蛋白的表达和分布。用肌动酶预处理的福尔马林固定组织，采用抗人腱生蛋白单克隆抗体（RBC1），通过抗生物素蛋白-生物素复合物法进行处理。在正常乳腺组织中，腱生蛋白分布于导管和小导管周围，但腺泡周围未见分布。在癌组织中，除小叶癌发病率较低（25%）外，各种组织学表现的腱生蛋白阳性病例发生率较高（大于67%）。与正常乳腺组织相比，癌灶周围可见强染色，但腱生蛋白常在癌间质交界处表达，此处有致密的纤维性间质，而在伴有活跃炎症改变和疏松纤维性间质的交界处则不表达。腱生蛋白的表达并非乳腺恶性肿瘤的全或无标记，其染色模式提示其在刺激癌细胞或伴随对癌细胞的促纤维增生反应的宿主防御机制中发挥作用。
