前列腺促甲状腺激素释放激素样肽的表达在不同物种之间以及恶性和非恶性组织之间存在差异。

Expression of prostatic TRH-like peptides differs between species and between malignant and nonmalignant tissues.

作者信息

Gkonos P J, Kwok C K, Block N L, Roos B A

机构信息

Geriatric Research, Education, and Clinical Center, Miami VA Medical Center, Florida.

出版信息

Prostate. 1993;23(2):135-47. doi: 10.1002/pros.2990230207.

DOI:10.1002/pros.2990230207

PMID:7690955

Abstract

Thyrotropin-releasing hormone-immunoreactive peptides (iTRH) were analyzed in normal rat and rabbit prostates and in human benign prostatic hyperplasia (BPH) and prostate cancer. Peptides were extracted from tissues, fractionated by anion and cation exchange chromatography, and analyzed by TRH radioimmunoassay. pGlu-Glu-Pro-NH2 predominated in rabbit, but accounted for only 10-15% of iTRH in rat and human BPH. Uncharged peptides predominated in rat and human prostate. Authentic TRH (pGlu-His-Pro-NH2) is not present in rabbit prostate, but may account for up to 25% of iTRH in rat and human prostate. iTRH was virtually absent in prostate cancer. These results demonstrate considerable heterogeneity in the expression of TRH-like peptides in the prostates of various animal species, and suggest decreased expression of these peptides in prostate cancer.

摘要

对正常大鼠和兔的前列腺以及人类良性前列腺增生（BPH）和前列腺癌中的促甲状腺激素释放激素免疫反应性肽（iTRH）进行了分析。从组织中提取肽，通过阴离子和阳离子交换色谱进行分离，并用促甲状腺激素释放激素放射免疫分析法进行分析。pGlu-Glu-Pro-NH2在兔中占主导，但在大鼠和人类BPH中仅占iTRH的10-15%。不带电荷的肽在大鼠和人类前列腺中占主导。正宗的促甲状腺激素释放激素（pGlu-His-Pro-NH2）在兔前列腺中不存在，但在大鼠和人类前列腺中可能占iTRH的25%。前列腺癌中几乎不存在iTRH。这些结果表明，不同动物物种前列腺中促甲状腺激素释放激素样肽的表达存在相当大的异质性，并提示这些肽在前列腺癌中的表达降低。