Alfthan H, Björses U M, Tiitinen A, Stenman U H
Helsinki University Central Hospital, Department of Obstetrics and Gynecology, FI.
Scand J Clin Lab Invest Suppl. 1993;216:105-13.
Pregnancy tests have sufficiently low detection limits (25-200 IU/L of human chorionic gonadotropin, hCG) and good specificity as judged by lack of cross-reaction with lutropin (LH) and follitropin (FSH). However, little information is available on the specificity of the tests for subunits and fragments of hCG. The dominating form of hCG immunoreactivity in pregnancy urine is actually a 10 kD fragment of the beta chain of hCG called the core fragment. We have evaluated ten commonly used pregnancy test with respect to specificity for various forms of hCG and detection limit. Our results show that the detection limits of the tests are close to that claimed by the manufacturers and that the main form of hCG immunoreactivity detected is intact hCG. Four of the tests also measure the free beta subunit of hCG and one method also the hCG beta core fragment, but the detection limits were 7-70 fold those for hCG.
妊娠试验的检测限足够低（人绒毛膜促性腺激素，即hCG为25 - 200 IU/L），且根据与促黄体生成素（LH）和促卵泡生成素（FSH）无交叉反应判断，其具有良好的特异性。然而，关于这些试验对hCG亚基和片段的特异性的信息却很少。妊娠尿液中hCG免疫反应性的主要形式实际上是hCGβ链的一个10 kD片段，称为核心片段。我们针对各种形式的hCG的特异性和检测限，对十种常用的妊娠试验进行了评估。我们的结果表明，这些试验的检测限接近制造商所宣称的，并且检测到的hCG免疫反应性的主要形式是完整的hCG。其中四种试验还能检测hCG的游离β亚基，一种方法还能检测hCGβ核心片段，但它们的检测限是hCG检测限的7 - 70倍。
