Atayar Cigdem, Poppema Sibrand, Visser Lydia, van den Berg Anke
Department of Pathology and Laboratory Medicine, University Medical Centre Groningen, 9700 RB Groningen, The Netherlands.
J Pathol. 2006 Feb;208(3):423-30. doi: 10.1002/path.1894.
Little is known about the cytokine profile of nodular lymphocyte predominance Hodgkin's lymphoma (NLPHL) and the significance of the characteristic rosetting CD4(+)/CD57(+) T cells. We analysed the T lymphocyte populations isolated from lymph node suspensions from five patients with NLPHL, two with follicular hyperplasia and progressive transformation of germinal centres (PTGC), three with classical Hodgkin's lymphoma (CHL) and five with hyperplasia of the tonsil. We sorted the T cells based on expression of CD3, CD4 and CD57 by flow cytometry and evaluated the cytokine mRNA profiles of the T cells with quantitative RT-PCR. NLPHL cases were as rich in T cells as the CHL cases, but all NLPHL cases had a much higher frequency of CD4(+)/CD57(+) T cells. In contrast to the CD4(+)/CD57(+) T cells from tonsils, IL2 and IL4 mRNAs were consistently absent from the CD4(+)/CD57(+) T cells of NLPHL. Even after stimulation, no IL4 transcripts could be detected in the CD4(+)/CD57(+) T cells of NLPHL. On the other hand, IFNgamma transcripts were elevated in NLPHL and PTGC T cell subsets as compared to tonsillar T cell subsets. IL13 mRNA was exclusively produced by the T cells of CHL cases, indicating that IL13 may be a key cytokine in CHL. In conclusion, elevated levels of CD4(+)/CD57(+) T cells are characteristic of NLPHL and these T cells display a distinct cytokine mRNA profile.
关于结节性淋巴细胞为主型霍奇金淋巴瘤（NLPHL）的细胞因子谱以及特征性玫瑰花结形成的CD4(+)/CD57(+) T细胞的意义，人们了解甚少。我们分析了从5例NLPHL患者、2例伴有生发中心滤泡增生和进行性转化（PTGC）的患者、3例经典型霍奇金淋巴瘤（CHL）患者以及5例扁桃体增生患者的淋巴结悬液中分离出的T淋巴细胞群体。我们通过流式细胞术根据CD3、CD4和CD57的表达对T细胞进行分选，并通过定量逆转录聚合酶链反应评估T细胞的细胞因子mRNA谱。NLPHL病例的T细胞数量与CHL病例一样丰富，但所有NLPHL病例中CD4(+)/CD57(+) T细胞的频率要高得多。与扁桃体的CD4(+)/CD57(+) T细胞不同，NLPHL的CD4(+)/CD57(+) T细胞中始终不存在IL2和IL4 mRNA。即使经过刺激，NLPHL的CD4(+)/CD57(+) T细胞中也检测不到IL4转录本。另一方面，与扁桃体T细胞亚群相比，NLPHL和PTGC的T细胞亚群中IFNγ转录本升高。IL13 mRNA仅由CHL病例的T细胞产生，表明IL13可能是CHL中的关键细胞因子。总之，CD4(+)/CD57(+) T细胞水平升高是NLPHL的特征，并且这些T细胞表现出独特的细胞因子mRNA谱。
