Tricoli J V, Yao J L, D'Souza S A, Bracken R B
Department of Surgery/Division of Urology, University of Cincinnati College of Medicine, Ohio 45267-0589.
Genes Chromosomes Cancer. 1993 Sep;8(1):28-33. doi: 10.1002/gcc.2870080106.
The human sex-region Y (SRY) gene maps to Yp11.3 and encodes a protein that shares significant sequence homology with a conserved DNA binding motif found in the nonhistone high-mobility group (HMG) proteins. In the mouse, Sry is required for normal testicular development and is expressed in the developing male gonadal ridge as well as in the adult testis. In man, SRY expression has been observed in the adult testis, but not in other adult male tissues. We have analyzed samples from human prostate adenocarcinoma and benign prostatic hypertrophy (BPH) for the expression of the SRY gene. We found expression of SRY in 60% of malignant prostate tumors and in three of six samples of BPH. We did not find expression in male or female colon mucosa, or in tissue from a cystic ovary. Malignant and atrophic testicular tissue both contained SRY transcript and served as positive controls in these experiments. We also found SRY transcript in the DU-145 prostate adenocarcinoma cell line. Interestingly, SRY expression is absent in the Tera-2 teratocarcinoma cell line. The potential for the SRY gene product to bind HMG core response elements in vitro suggests that SRY could participate in the cascade of gene regulatory events that result in aberrant cell growth or malignancy.
人类Y染色体性别决定区（SRY）基因定位于Yp11.3，编码一种蛋白质，该蛋白质与非组蛋白高迁移率族（HMG）蛋白中发现的保守DNA结合基序具有显著的序列同源性。在小鼠中，Sry是正常睾丸发育所必需的，在发育中的雄性性腺嵴以及成年睾丸中表达。在人类中，已在成年睾丸中观察到SRY表达，但在其他成年男性组织中未观察到。我们分析了来自人类前列腺腺癌和良性前列腺增生（BPH）的样本中SRY基因的表达情况。我们发现60%的恶性前列腺肿瘤以及6个BPH样本中的3个样本中有SRY表达。我们在男性或女性结肠黏膜以及囊性卵巢组织中未发现表达。恶性和萎缩性睾丸组织均含有SRY转录本，并在这些实验中作为阳性对照。我们还在DU-145前列腺腺癌细胞系中发现了SRY转录本。有趣的是，在Tera-2畸胎癌细胞系中未发现SRY表达。SRY基因产物在体外结合HMG核心反应元件的可能性表明，SRY可能参与导致异常细胞生长或恶性肿瘤的基因调控事件级联反应。
