Coromili V, Chang T M
Artificial Cells and Organs Research Centre, McGill University, Montreal, Canada.
Biomater Artif Cells Immobilization Biotechnol. 1993;21(3):427-44. doi: 10.3109/10731199309117382.
Applications of alginate polylysine (APL) microcapsules in cell culture engineering and hybrid artificial organs require strict control of membrane permeability. For example, hybrid artificial organs must permit the diffusion of smaller molecules including peptides and proteins and the exclusion of leucocytes and immunoglobulins (MW > 150,000). Single molecular weight solutes such as proteins have been used to study the molecular weight cut-off of the membrane. A new approach that uses a heterogenous mixture of dextrans of different molecular weights: -MW 10,000-500,000 as a test solute in diffusion experiments is described. Intra/extra capsular changes in concentration and molecular weight distribution of the heterogenous dextran mixture are monitored and determined by high performance gel chromatography. MW can then be related to diffusional Stokes radius, a more useful parameter when comparing permeability of cell membrane to different test solutes.
海藻酸钠-聚赖氨酸（APL）微胶囊在细胞培养工程和混合人工器官中的应用需要严格控制膜的通透性。例如，混合人工器官必须允许包括肽和蛋白质在内的较小分子扩散，并排除白细胞和免疫球蛋白（分子量>150,000）。单一分子量的溶质如蛋白质已被用于研究膜的截留分子量。本文描述了一种新方法，即在扩散实验中使用不同分子量（10,000 - 500,000）的葡聚糖异质混合物作为测试溶质。通过高效凝胶色谱法监测并确定异质葡聚糖混合物在囊内/囊外的浓度和分子量分布变化。然后，分子量可以与扩散斯托克斯半径相关联，在比较细胞膜对不同测试溶质的通透性时，扩散斯托克斯半径是一个更有用的参数。
