Gilmour N J, Angus K W
Res Vet Sci. 1976 Jan;20(1):6-9.
In five calves experimentally infected with Mycobacterium avium and in 10 with M johnei it was shown that the serological response by the fluorescent antibody (FA) test was specific. The serological response in the M avium infected calves was transitory and lasted up to five months. The first evidence of a serological response with the FA test in the M johnei dosed calves occurred at four months, and all calves had reacted by nine months after dosing. At the 23 tests carried out between four and 32 months after dosing, when the experiment was terminated, an average of 5-8 animals was positive at any one test. Johne's disease was confirmed bacteriologically and histologically in six of the 10 calves though none had shown clinical signs.
在5头经实验感染鸟分枝杆菌的小牛和10头感染副结核分枝杆菌的小牛中，荧光抗体（FA）试验显示血清学反应具有特异性。感染鸟分枝杆菌的小牛血清学反应是暂时的，持续长达5个月。在接种副结核分枝杆菌的小牛中，FA试验血清学反应的首个证据出现在4个月时，所有小牛在接种后9个月时均已出现反应。在接种后4至32个月（实验终止时）进行的23次检测中，每次检测平均有5 - 8只动物呈阳性。10头小牛中有6头经细菌学和组织学确诊患有副结核病，尽管它们均未表现出临床症状。
