de Lisle G W, Samagh B S, Duncan J R
Can J Comp Med. 1980 Apr;44(2):183-91.
Fecal culture for Mycobacterium paratuberculosis and a complement fixing serological test using a carbohydrate antigen were compared for diagnostic efficiency in cattle naturally infected with M. paratuberculosis. Serological reactivity was associated with the persistent fecal shedding of large numbers of bacteria and in the absence of false positives in the population studied, was considered an efficient method for the identification of this segment of infected cattle. Minimally infected cattle, which inconsistently shed small numbers of bacteria, were generally serologically negative. One persistent fecal shedder demonstrated a fluctuating serological response to the carbohydrate antigen following abortion. The advantages and disadvantages of this serological diagnostic procedure in relationship to fecal culture are discussed.
对自然感染副结核分枝杆菌的牛，比较了副结核分枝杆菌粪便培养和使用碳水化合物抗原的补体结合血清学检测的诊断效率。血清学反应性与大量细菌的持续粪便排菌有关，在所研究的群体中没有假阳性，被认为是识别这部分感染牛的有效方法。轻度感染的牛，排菌数量少且不持续，通常血清学呈阴性。一头持续粪便排菌牛在流产后对碳水化合物抗原的血清学反应呈波动状态。讨论了这种血清学诊断方法相对于粪便培养的优缺点。
