Miyahara H, Hotokebuchi T, Saikawa I, Arita C, Takagishi K, Sugioka Y
Department of Orthopedic Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Clin Immunol Immunopathol. 1993 Oct;69(1):69-76. doi: 10.1006/clin.1993.1151.
Type II collagen-induced arthritis (CIA) is a pathologic process mediated, in part, by humoral immune mechanisms. Because many antibody-mediated reactions are neutrophil-dependent, the role of this cell population was examined in passive CIA transferred with anti-type II collagen (CII) antibody. In cyclophosphamide (CY)-induced leukocytopenic rats, swelling and inflammation associated with the arthritic response were significantly reduced. Concomitant administration of recombinant human granulocyte colony-stimulating factor (rhG-CSF) to leukocytopenic rats for 7 consecutive days from the day of CY injection resulted in the recovery of peripheral blood neutrophils count and the abrogation of the suppression of arthritis with an optimal dose of anti-CII antibody. Further study demonstrated that a prior administration of rhG-CSF to naive rats for five consecutive days resulted in the significant and specific increase of peripheral blood neutrophils count and enhancement of passive arthritis with a suboptimal dose of anti-CII antibody. It was suggested that neutrophils played an important role in the development of passive CIA.
II型胶原诱导的关节炎（CIA）是一种部分由体液免疫机制介导的病理过程。由于许多抗体介导的反应依赖中性粒细胞，因此在以抗II型胶原（CII）抗体被动转移的CIA中研究了该细胞群体的作用。在环磷酰胺（CY）诱导的白细胞减少症大鼠中，与关节炎反应相关的肿胀和炎症明显减轻。从注射CY之日起连续7天给白细胞减少症大鼠同时注射重组人粒细胞集落刺激因子（rhG-CSF），可使外周血中性粒细胞计数恢复，并消除最佳剂量抗CII抗体对关节炎的抑制作用。进一步的研究表明，对未免疫大鼠连续5天预先给予rhG-CSF，可使外周血中性粒细胞计数显著且特异性增加，并增强次优剂量抗CII抗体诱导的被动性关节炎。提示中性粒细胞在被动性CIA的发展中起重要作用。
