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利用荧光诱导法测定海洋甲藻米氏原甲藻中对硫磷的毒性:一种生物检测的可能模型。

Fluorescence induction used to measure parathion toxicity in the marine dinoflagellate Prorocentrum micans: a possible model for biodetection.

作者信息

Prevot P, Perret E, Jupin H, Soyer-Gobillard M O

机构信息

Département de Biologie Cellulaire et Moléculaire, Laboratoire Arago, Université Pierre et Marie Curie, CNRS UA 117, Banyuls sur Mer, France.

出版信息

Ecotoxicol Environ Saf. 1993 Jun;25(3):360-71. doi: 10.1006/eesa.1993.1032.

Abstract

The marine dinoflagellate Prorocentrum micans Ehrenberg was used as a test organism to determine the conditions of use of fluorescence induction kinetic measurements in the study of parathion phytotoxicity. Measurements were taken of the kinetics of slow and fast fluorescence induction in whole cells and isolated chloroplast fragments at various concentrations of parathion. In both types of induction, the addition of parathion greatly decreased the fluorescence yield, indicating either inhibition of electron transport or physical changes. The action of parathion was a function of its concentration and was similar to the action of DCMU. Fluorescence induction was clearly less in isolated chloroplast fragments than in whole cells.

摘要

海洋双鞭毛藻微小原甲藻(Prorocentrum micans Ehrenberg)被用作测试生物,以确定在对硫磷植物毒性研究中使用荧光诱导动力学测量的条件。在不同浓度的对硫磷下,对全细胞和分离的叶绿体片段中慢速和快速荧光诱导的动力学进行了测量。在两种诱导类型中,对硫磷的添加都大大降低了荧光产量,这表明电子传递受到抑制或发生了物理变化。对硫磷的作用是其浓度的函数,并且与敌草隆(DCMU)的作用相似。分离的叶绿体片段中的荧光诱导明显低于全细胞中的荧光诱导。

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